Preparation and biocompatibility of panax notoginseng saponins-hydroxyapatite/ chitosan scaffold

doi:10.3969/j.issn.2095-4344.2013.34.009

Abstract

Abstract:

BACKGROUND: Panax notoginseng saponins promotes bone repair by improving vascular proliferation. Therefore, the scaffolds carrying panax notoginseng saponins were supposed to be used to improve bone repair at the bone defect region. However, the biocompatibility of scaffolds remains unclear.

OBJECTIVE: To evaluate the biocompatibility of panax notoginseng saponins-hydroxyapatite/chitosan scaffolds.

METHODS: A new bone repair scaffold has been generated by thoroughly mixtures of 0, 0.1, 1, 10 mg panax notoginseng saponins and chitosan/hydroxyapatite using in-situ composite technique and freeze-drying technique. Morphology and mechanical property of the scaffold were observed under a scanning electron microscope. (1) Cell proliferation test: rabbit bone marrow mesenchymal stem cells of passage 3 were cultured in four kinds of drug loaded hydroxyapatite/chitosan composite material leaching liquor. Cells normally cultured were considered as controls. 3-(4, 5-Dimethylthiazol-2-yl) 2, 5-diphenyl tetrazolium bromide was used to measure absorbance value of cells in each group. (2) Hemolysis test: Rabbit anticoagulated blood was added with four kinds of drug loaded hydroxyapatite/chitosan composite material leaching liquor. Absorbance values were measured using a microplate reader in each group. (3) Pyrogen test: The four kinds of drug loaded hydroxyapatite/chitosan composite material leaching liquor and saline were respectively injected into ear vein of rabbits, and the increase of rabbit body temperature was detected.

RESULTS AND CONCLUSION: Drug loaded hydroxyapatite/chitosan composite material contained three dimensional porous structure of 110 μm in diameter. Drug loading process of panax notoginseng saponins did not significantly affect the porosity, pore size and density of the composite material, but decreased its breaking strength and elastic modulus. The larger amount of drug loading showed a more obvious effect. Simple hydroxyapatite/chitosan composite material had good cellular compatibility. The composite material after drug loading obviously suppressed cell proliferation, and the larger amount of drug loading showed a more obvious effect. The composite material had good blood compatibility before and after drug loading. The composite material had good pyrogen effects before and after drug loading, but accorded with acceptable quality level of pyrogen test.

Key words: biomaterials, material biocompatibility, panax notoginseng saponins, hydroxyapatite, chitosan, scaffold, biocompatibility, bone tissue engineering, provincial grants-supported paper

CLC Number:

R318

Chen Shu-xiang, Kang Le, Ou Wen-huan, Si Tu-jian, Ding Lin-jian. Preparation and biocompatibility of panax notoginseng saponins-hydroxyapatite/ chitosan scaffold[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(34): 6116-6122.

Figures/Tables 4

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